Background 

What is Cell Painting?

Cell Painting (CP) is a high-content, image-based morphological profiling assay. Up to six fluorescent dyes are used, together revealing eight cellular components or organelles. Combined with sophisticated image analysis tools, this approach allows the user to obtain detailed cellular profiles, consisting of hundreds or thousands of morphological features. The amount of detail in these datasets makes this assay sensitive to subtle cellular changes, and therefore a valuable tool to assess cellular responses to drugs or genetic perturbations. 

What is JUMP-CP?

The Joint Undertaking in Morphological Profiling (JUMP) Cell Painting (CP) consortium, spearheaded by the Broad Institute of MIT and Harvard, was established to create an unprecedented public reference Cell Painting data set. They developed a standardized Cell Painting protocol, which was used to obtain the phenotypic profiles of over one billion cells responding to over 140.000 small molecules and genetic perturbations. Read more on the JUMP-CP Consortium website. 

Further reading

To learn more about the ZEISS arivis software, visit the ZEISS website.